Journal: The Journal of Neuroscience

Article Title: Enhanced Excitability of Thalamic Sensory Neurons and Slow-Wave EEG Pattern after Stimuli That Induce Spinal Long-Term Potentiation

doi: 10.1523/JNEUROSCI.2110-13.2013

Figure Lengend Snippet: A, B, Oscilloscope traces of innocuous (brush) and noxious (pinch, C-fiber stimulation of sciatic nerve) stimuli on spike activity of a thalamic VPL neuron before (A) and after HFS (B). Note the characteristic spike responses after each stimulus. Following sham, brush and pinch responses were indistinguishable versus their control response. High-frequency after-discharges occurred following each stimulus type after HFS. C, D, Histogram plots depicting the time course effect of sham HFS (C) and HFS (D) on brush, pinch, and sciatic nerve “C-fiber” stimulus-evoked response magnitude of thalamic VPL neurons. Each histogram bar represents the group mean (±SEM) response in spikes per stimulus application at different time points for all VPL neurons tested (n = 40). Note the marked and sustained enhancement in innocuous and noxious stimulus-induced VPL response magnitude following HFS (n = 30 neurons in 15 rats) but not sham HFS (n = 10 neurons in 7 rats, *p < 0.05, **p < 0.01, one-way ANOVA, Dunn's multiple-comparison test).

Article Snippet: The amplified signal was then routed to an oscilloscope (Model D11; Tektronix) and a spike processor (Model D130; Digitimer; , WD), which was manually set to detect only the VPL neuron action potential waveforms.

Techniques: Activity Assay, Control, Comparison

Journal: The Journal of Neuroscience

Article Title: Enhanced Excitability of Thalamic Sensory Neurons and Slow-Wave EEG Pattern after Stimuli That Induce Spinal Long-Term Potentiation

doi: 10.1523/JNEUROSCI.2110-13.2013

Figure Lengend Snippet: HFS enhancement of GLU-evoked excitations of thalamic VPL neurons. A, The top left traces represent oscilloscope traces of a single VPL neuron‘s spike activity and spike template recorded in spike acquisition software during a single GLU pulse ejection. B, The rate meter traces of pulsatile GLU ejections on the same VPL neuron over a 5 min period before and 30 min after HFS. Note the marked increase in consecutive GLU-evoked responses following HFS. The overall time course of the HFS effect on GLU response magnitude overlapped with that for pinch, brush, and C-fiber evoked responses, see Figure 2. C, The time expanded rate-meter plots reflect computer-averaged (bin by bin) plots of the eight individual GLU-responses in B. E, HFS also significantly shortened the 50% time to maximum response (E) and steepened the slope of the rising phase (G) of excitation by juxtacellular glutamate in this neuron. The histogram bars in D, F, and H represent the group mean (±SEM) average magnitude, 50% time to max and slope of the response onset to GLU, respectively, for 10 VPL neurons in six rats before and after HFS (*p < 0.05, Mann–Whitney rank sum test).

Article Snippet: The amplified signal was then routed to an oscilloscope (Model D11; Tektronix) and a spike processor (Model D130; Digitimer; , WD), which was manually set to detect only the VPL neuron action potential waveforms.

Techniques: Activity Assay, Software, MANN-WHITNEY